Tattvagene

Tattvagene

A Successful Pregnancy Beings With A Healthy Embryo

Tattvagene is a specialised genetic testing laboratory whose mission is to assist in increaing the chance of having a successful pregnancy and a healthy child. The centre was established 2015, with an aim to offer reliable genetic tests using state-of-the-art technology that would address various needs especially in the Assisted Reproductive Technology (ART) field.
  • Parental Karyotyping & Genetic Mutation Tests (Y-chromosome microdeletion, Beta Thalassemia, Spinal Muscular Atrophy, Fragile X Syndrome, etc.)
  • Non-invesive Prenatal Screening (NIPS)
  • Preimplantation Genetic Screening (PGS)
  • Preimplantation Genetic Diagnosis (PGD)

Karyotyping

  • Karyotyping is most widely used preliminary test for unexpected infertility.
  • The test reveals structural aberrations and aneuploidy
  • A source of diagnostic information for genetic disorders and specific birth defects
  • This test may be done on a couple having a history of miscarriage or to examine a baby with unusual features or developmental delays
  • Recomended before receving assisted reproductive technology (ART)

Fluorescence In Situ Hybridization (FISH)

  • A molecular cytogenetic test used in localizing specific DNA sequences on chromosomes
  • Gene-specific probs are used to detect presence or absence of a particular gene in metaphase chromosomes/interphase cells
  • This helps to detect sub-microscopic chromosomal changes beyond the resolution of karyotyping

Prenatal Diagnosis

  • Prenatal karyotyping & FISH are recommended for cases of advanced maternal age, cases with an abnormal result from non-invasive screening, abnormal ultrasound findings or chromosomal aberrations in one of the parents
  • Analysis of cultured cells from the amniotic fluid, during the second trimester at 14 to 18 weeks of gestation determines the chromosomal constitution.
PGS

Preimplantation Genetic Screening

  • PGS is a diagnostic test used for reducing implantation failures in In Vitro Fertilization (INT) pregnancies
  • It helps in selection of normal embryos with a normal chromosome. complement and thus improving INT pregnancy and subsequent live birth rates

Technology

  • We use Next Generation Sequencing (NGS) technology to perform PGS
  • Detects aneuploidy and mosaicism in the embryos
  • Delivers highly detailed, accurate, and scalable. results for improved selection of euploid embryos
NGS

Next Generation Sequencing

Embryo Biopsy

NGS

Intra Cytoplasmic sperm injection with sucking and injecting pipette in position

PGD

Preimplantation Genetic Diagnosis

PGD Aims to reduce or eliminate the risk of inherited genetic conditions that either one or both the parents are carriers for.

In this procedure the gene that causes the monogenic disorder is sequenced to look for mutations in euploid embryos prior to INT transfer.

PGD with HLA matching

  • Beta thalassemia is one of the most common single-gene disorder
  • PGD Ivith HLA-typing for the selection of HLA-identical embryos, a therapeutic approach
  • This enables the parents to conceive a child who is healthy, as well as a perfect transplant match for their affected child
EMBRYO

Data Analysis & Reporting

Euploid Embryo

Embryo Transfer

NIPS

Non-Invasive Prenatal Screening

A new, safe and highly accurate method to detect common aneuploidies like Down spidrome, Edwards syndrome and Patau syndrome with a simple blood draw from the mother.

Technology

  • The test analyzes cell-free fetal DNA (cffDNA) which circulates freely in the maternal blood and is used to detect if your baby is at risk for certain genetic conditions
  • It is capable of genome-wide aneuploidy detection over the whole foetal genome DNA and gives results for all 23 pairs of chromosomes
  • This has a detection rate of 99% and is therefore considered very sensitive
Prenatal Aneuploidy Test

Sample Preparation

Screening

Trisomy 21
Trisomy 18
Trisomy 13
Sex chromosome Aneuploidy

Personal Risk Scoring

Trisomy 18 (Edwards Syndrome)
Trisomy 21 (Down Syndrome)
The test is capable of genome•ide aneuplady detection over the whole fetal genome (23 pairs of chromosomes) and offers an interpretation of the results for Trisorny 13 Trisomy 18 and Trisomy 21. Th is test confers an accuracy of up to 99% on the detection of fetal chromosome aneuploidy

Prenatal Aneuploidy Test Results of Chromosome 13,18 and 21

ChromosomeRiskTest Results
Chromosome 13
Palau Syndrome
-No aneuploidy detected
Chromosome 18
Edwards Syndrome
-No aneuploidy detected
Chromosome 21
Down Syndrome
-No aneuploidy detected
Tattvagene lab Test

Tattvagene lab Test Lists

Test CodeCategoryTestMethodSample type requiredTAT
TGK001CytogeneticsKaryotyping
GTG bandingPeripheral blood (PB)12 - 15 days
TGK002
CytogeneticsKarytyping + NOR StainingSilver stainingPeripheral blood (PB)12 - 15 days
TGK003CytogeneticsKarytyping + C-Banding

Giemsa stainingPeripheral blood (PB)12 - 15 days
TGY001
Parental screeningY Microdeletion - Azoospermia / Oligospermia
PCR/SangerPeripheral blood (PB)12 - 15 days
TGB002
Parental screeningβ-Thalassemia
PCR/SangerPeripheral blood (PB)12 - 15 days
TGF003
Parental screeningFragile X Screening
PCR/SangerPeripheral blood (PB)12 - 15 days
TGF004
Parental screeningFragile X CGG sizing
SangerPeripheral blood (PB)12 - 15 days
TGT005
Parental screeningTargeted Testing
PCR/SangerPeripheral blood (PB)12 - 15 days
TGS006Parental screeningXY - sex reversal -SRY by PCR
PCR/SangerPeripheral blood (PB)12 - 15 days
TGP007
Preimplantation screeningPre-Implantation Genetic Testing for aneuploidies (PGT-A) (per embryo)
NGSDay 5 Trophectoderm biopsy, 5-6 cells15 days
TGP008
Preimplantation screeningPre-Implantation Genetic Testing for monogenic disorder (PGT-M) (per embryo)
NGS/SangerDay 5 Trophectoderm biopsy, 5-6 cells15 days
TGP009Preimplantation screeningPGS + laser biopsy by our embryologist (per embryo)
NGSDay 5 Trophectoderm biopsy, 5-6 cells15 days
TGN010
PrenatalNon-Invasive Prenatal Screening (NIPS)
NGSPeripheral blood (PB)12 - 15 days
TGFS011
PrenatalFISH-13/18/21 - Amniotic fluid (10ml)
Fluorescent in situ hybridizationAmniotic fluid2 days
TGFS012
PrenatalFISH LSI 21 - Amniotic fluid (10ml)
Fluorescent in situ hybridizationAmniotic fluid2 days
TGFS013
PrenatalFISH LSI 13 - Amniotic fluid (10ml)
Fluorescent in situ hybridizationAmniotic fluid2 days
TGFS014PrenatalLSI 13/21 - Amniotic fluid (10ml)
Fluorescent in situ hybridizationAmniotic fluid2 days
TGFS015
Molecular CytogeneticsSex determining Region Y (SRY)gene by FISH
Fluorescent in situ hybridizationPeripheral blood (PB)8 days
TGFS016
Molecular CytogeneticsFISH - Prader-Willi/ Angelman Region Probe - Microdeletion
Fluorescent in situ hybridizationPeripheral blood (PB)12 days
TGFS017
Molecular CytogeneticsFISH - DiGeorge/ VCFS region probe - Microdeletion
Fluorescent in situ hybridizationPeripheral blood (PB)12 days
TGFS018
Molecular CytogeneticsFISH - DGS2 -10p14 - Microdeletion
Fluorescent in situ hybridizationPeripheral blood (PB)12 days
TGFS019
Molecular CytogeneticsFISH - TBX1 22q11.2 - Microdeletion
Fluorescent in situ hybridizationPeripheral blood (PB)12 days
TGFS020
Molecular CytogeneticsFISH - Tel Vysion 4p - Microdeletion
Fluorescent in situ hybridizationPeripheral blood (PB)12 days
TGFS021
Molecular CytogeneticsFISH - Tel Vysion 1p - Microdeletion
Fluorescent in situ hybridizationPeripheral blood (PB)12 days
TGFS022
Molecular CytogeneticsFISH - 1p36 - Microdeletion
Fluorescent in situ hybridizationPeripheral blood (PB)12 days
TGFS023
Molecular CytogeneticsFISH - Williams Syndrome - Microdeletion
Fluorescent in situ hybridizationPeripheral blood (PB)12 days
TGFS024
Molecular CytogeneticsFISH - Miller - Dieker Chromosome Region Probe (MDS)- Microdeletion
Fluorescent in situ hybridizationPeripheral blood (PB)12 days
TGFS025
Molecular CytogeneticsFISH - Smith - Magenis Chromosome Region Probe (SMS)- Microdeletion
Fluorescent in situ hybridizationPeripheral blood (PB)12 days
TGFS026
Molecular CytogeneticsFISH - Wolf-Hirschhorn syndrome (WHS) - Microdeletion
Fluorescent in situ hybridizationPeripheral blood (PB)12 days
TGFS027
Molecular CytogeneticsSpectral Karyotype (SKY)
Fluorescent in situ hybridizationPeripheral blood (PB)15 - 20 days
TGFS028Molecular CytogeneticsFISH - X/Y for post bone marrow transplant analysis
Fluorescent in situ hybridizationPeripheral blood (PB)4 days
TGFS029
Postnatal ScreeningGauchers Disease -6 common Mutations, exons 9 & 10
PCR/SangerPeripheral blood (PB)15 days
TGFS030
Postnatal ScreeningGauchers Disease -complete coding sequence
PCR/SangerPeripheral blood (PB)15 days
TGFS031
Postnatal ScreeningGlycogen Storage Disease - 1a
PCR/SangerPeripheral blood (PB)15 days
TGFS032
Postnatal ScreeningGlycogen Storage Disease - 1b
PCR/SangerPeripheral blood (PB)15 days
TGFS033
Postnatal ScreeningNon Syndromic Hearing Loss - connexin 26
PCR/SangerPeripheral blood (PB)15 days
TGFS034
Postnatal ScreeningNoonans Syndrome NGS targetted gene panel
PCR/SangerPeripheral blood (PB)15 days
TGFS035
Postnatal ScreeningAchondroplasia -common mutation 1138 G>A/ 1138 G>C
PCR/SangerPeripheral blood (PB)15 days
TGFS036
Postnatal ScreeningHLA typing (6 Loci)NGSPeripheral blood (PB)25 - 30 days
TGFS037Postnatal ScreeningSpinal muscular atrophy / SMAPCR/SangerPeripheral blood (PB)15 days
Tattvagene lab Test

Tattvagene lab Test Lists